Comparative study of domestic and imported bilobalide in the treatment of Pneumocystis carinii pneumonia in rats - Master's thesis - Dissertation

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Pneumocystis carinii pneumonia (PCP) is more common in immunocompromised or defective populations. With the expansion of high-risk groups such as cancer chemotherapy patients, organ transplant recipients treated with immunosuppressive agents, autoimmune patients, and patients with acquired immunodeficiency, especially in the first AIDS epidemic in 1984, the incidence of PCP has increased dramatically. the trend of. The main drugs currently used to prevent and treat PCP are sulfamethoxazole (TMP-SMZ), pentamidine and the like. Although these drugs can receive satisfactory results, but there are serious adverse reactions or drug resistance, it is an important research topic to find new drugs to prevent and treat PCP. The experiment confirmed that

It has a certain therapeutic effect on PCP [1]. Authors compare domestic and imported

To treat the efficacy of PCP, the results are reported below.

Materials and Methods

l Establishment of PCP

70 pure Wistar rats, female, weighing 230-270 g, were provided by Chongqing Medical Science Experimental Animal Center (license number: Sichuan Medical Laboratory Animal Management Committee, No. 2 4301045). The experimental rat pneumocystis pneumonia model was established according to the literature [1] method.

2 Experimental rats grouping and treatment

PCP rats were randomly divided into 4 groups, 16 in each group. Group A: immunosuppressed rat control group, no drug treatment was given; group B: domestic

(Guizhou Aofang Biotechnology Co., Ltd., batch number 010405, 95 HPLC, powder) treatment group, 20 mg/kg·d×8 d intraperitoneal injection; Group C: import

(Sigma, batch 053K1397, 95 HPLC, powder) treatment group, 20 mg / kg · d × 8 d intraperitoneal injection; group D: compound sulfamethoxazole (Southwest Pharmaceutical Co., Ltd., batch number 020503, SMZ: TMP =5:1, tablet) The control group received SMZ 100 mg/kg·d×8 d+TMP20 mg/kg·d×8 d. After 8 days of continuous administration, the drug was stopped for 1 week, and the efficacy was examined at the 9th week.

3 efficacy assessment

During the course of treatment, the survival status of each group of rats was observed, and the number of survivors was recorded and weighed weekly. At the completion of the experiment, the rats were taken from the lungs, the trachea and hilar tissues were removed, and the surface moisture was absorbed, and the whole lung wet weight was weighed. The five lung leaves of each mouse were cut up and down, and each face was scattered on the same slide. Tablets, air drying, methanol fixation, GMS staining, oil smear observation, 100 fields of view were sequentially observed for each lung leaf print, the number of cysts of Pneumocystis was counted, and the average number of capsules per field per lung and the package of each group of rats were calculated. The average number of capsules.

4 statistical analysis

The body weight, lung weight, lung weight/body weight ratio of the rats in each experimental group, and the mean number of cysts per field of lung prints were compared by analysis of variance. The reduction rate of cysts in each treatment group was calculated according to the following formula:

1 curative effect

1.1 Animal survival status At the end of the experiment, 2 rats died in group A, 1 in group C, and no death in group B and group D.

1.2 Changes in body weight of rats The changes in body weight before and after treatment in each group of rats are shown in Table 1. The average body weight of rats in group A decreased, and the weight of rats in the other treatment groups increased to varying degrees. Statistical analysis showed that the difference in the average body weight of the rats in group A was significantly different from that in the other treatment groups (P<0.05). Domestic and imported

There was no significant difference in group (P>O.05).

1.3 Rat lung weight, lung weight / body weight ratio The lung weight and lung weight / body weight ratio of the rats in each experimental group at the end of the experiment are shown in Table 2. The analysis found that the lung weight and lung weight/body weight ratio of the control group were significantly higher than the treatment group (P < 0.05);

Group and import

There was no significant difference in the group (P>0.05).

1.4 Number of cysts in lung prints The average number of cysts in each field of lung prints of each group was shown in Table 3. The average number of cysts per field in the treatment group was lower than that in the control group.