GAPDH Antibodies - How to choose the right GAPDH internal reference antibody? -Huaqiang Electronic Network

In Western blot experiments, the most used is the internal reference antibody. As a reference system that plays an important role, as long as you start a Western blot, no matter what protein, you must use an internal reference antibody. Here, Amy Jie strongly recommends GAPDH. Bull papers published in foreign big-name magazines generally use GAPDH internal reference. What the big cattle lab uses, certainly not wrong. AMD's Abbkine brand of GAPDH antibodies. Because the price is really high. The following is a shared experience of Wu University students as an old customer to do Western blot. Because the molecular weight of the target protein was about 50kD, the previous β-Tubulin was not very suitable. I bought an Abbkine GAPDH antibody as an internal reference. The titer was a bit surprising. I used a 1:1000-fold initial dilution to think that the band was very Thick, and later diluted to 1:8000, while reducing the exposure time, the strip is still very clear. ——One client of the Life Science Institute of Wuhan University How to choose the right GAPDH antibody? Although there are many choices of internal reference antibodies, they also need to follow certain principles and meet the practical application requirements. First, we need to consider the size of the protein of interest. We recommend that the internal reference should have a molecular weight difference of more than 5KD from the target protein to be detected. Therefore, you need to select the appropriate internal reference according to the molecular weight of the protein you are trying to detect, because the molecular weight of GAPDH is about 36 kD, therefore the internal reference antibody is not well suited for use in WB assays for detecting target proteins of 30 kD-40 kD size. In addition, there are certain differences in the selection of internal reference samples from different tissue sources. For example, in some cells, due to tissue hypoxia, diabetes and other factors, the expression of GAPDH is increased, which is not suitable for internal reference.

Product name and description

Item number

specification

product manual

Anti-

GAPDH Mouse Monoclonal Antibody (2B5)

A01020

50ul/1ml

Details

GAPDH Polyclonal Antibody

ABP50152

30ul/200ul

Details

HRP Conjugated Anti-GAPDH

Mouse

Monoclonal Antibody

( 2B5)

A01025

50ul/1ml

Details

Abbkine also offers a large package of 1ml of GAPDH internal reference antibody, which is ideal for routine internal validation or calibration, or for immunological experiments in cytoskeletal fluorescence localization. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during the use, please contact your local agent Ai Meijie Technology Co., Ltd. directly.

A

The bbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Introducing it to China as a regional agent in China, BBKine will provide customers with the most cost-effective routine immunological reagents, such as various Label antibodies, internal reference antibodies, conventional primary antibodies, and a full range of secondary antibodies, antibody detection substrates, and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line.

400-6800-868

Contact Amytech Co., Ltd. for product information and complete experimental solutions.