The enzyme-linked immunosorbent assay (ELISA) kit is one of the most widely used methods for drug residue detection. Compared to instrumental analysis, it offers advantages such as speed, simplicity, accuracy, and high sensitivity. However, ELISA requires strict experimental conditions, including both the laboratory environment and the operator's skill level. Despite this, the ELISA kit has relatively lower requirements for the lab environment compared to other drug residue detection techniques.
Since most of the testing procedures are manually performed by the experimenter, human error can significantly affect the results. Based on several years of hands-on experience, I would like to share some key points to consider when performing ELISA tests.
One common issue is when the test result shows no absorbance or very low absorbance. This may be due to an expired kit, using reagents from different batches, forgetting to add the enzyme label or reagent, or not allowing the reagents to warm up properly. Additionally, if the incubation temperature is too low, it could also lead to poor results. To resolve this, always use kits within their expiration date and ensure all reagents are from the same batch. Follow the instructions in the kit carefully, allow the reagents to reach room temperature before use, and monitor the incubator temperature throughout the experiment.
Another important aspect is the use of negative and positive controls. If both the positive and negative controls show expected results, it indicates that the test was conducted correctly. The blank control, which involves no sample and no enzyme, helps measure the background absorbance of the system. It is especially critical when working with single wavelengths.
Our company provides a wide range of ELISA kits, thousands of antibodies, and nearly 10,000 top-tier products across fields such as molecular biology, immunology, and cell biology. We also offer testing services to save you time and effort.
If you're interested in learning more about ELISA or need assistance with your experiments, feel free to reach out. We’re here to help you achieve accurate and reliable results every time.
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