Proteomics research plays a crucial role in understanding the molecular mechanisms of life and provides a foundation for solving complex disease processes. Since genes can produce multiple protein variants through post-translational modifications, the same gene may result in different proteins under varying conditions, times, and locations. This high complexity and variability in protein expression make proteomics studies highly challenging.
Therefore, any technology that can significantly reduce sample complexity will greatly enhance the efficiency and accuracy of proteomic analysis. The Agilent 3100 OFFGEL isoelectric focusing system is one such powerful tool designed to simplify protein samples before downstream analysis, improving both sensitivity and resolution.
The technical principle of the Agilent 3100 OFFGEL involves the separation of proteins or peptides based on their isoelectric points (pI). When a sample is placed on an immobilized pH gradient (IPG) strip, it migrates within the pH range of the strip under an electric field. This process allows for stepwise isoelectric focusing, with the final sample remaining in the upper buffer, ready for collection and further analysis.
The main application of the Agilent 3100 OFFGEL lies in its ability to pre-separate protein samples by pI, which reduces complexity and enhances detection sensitivity. It works seamlessly with other key technologies in proteomics, such as two-dimensional gel electrophoresis, mass spectrometry, and bioinformatics. With a pH resolution of 0.1–0.6, the system enables precise fractionation, making it ideal for LC/MS workflows.
In addition to its scientific applications, this equipment is widely used in various biological and medical research fields. From ELISA kits to zymography tests, companies like Nanjing Xinfan Biotechnology Co., Ltd. provide a comprehensive range of reagents and tools to support advanced research and diagnostic needs.
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