Proteomics plays a crucial role in understanding the molecular basis of life processes and provides essential insights into the mechanisms behind various diseases. Since gene expression often leads to the production of protein precursors, which are then processed and modified into biologically active forms, the same gene can produce multiple proteins under different conditions, times, or locations. This complexity and variability in protein expression make proteomics research both challenging and fascinating.
To overcome these challenges, technologies that simplify protein sample complexity are highly valuable. The Agilent 3100 OFFGEL isoelectric focusing separator is one such tool that significantly reduces the complexity of protein samples, enhancing the sensitivity and resolution of downstream analyses. This makes it an essential instrument in modern proteomics workflows.
3100 OFFGEL Technical Principle
The Agilent 3100 OFFGEL separates proteins or peptides based on their isoelectric points (pI). When a sample is placed on a pre-cast pH gradient strip (IPG) with buffer, an electric field causes the proteins to migrate through the IPG medium. They stop at their respective pI values, allowing for stepwise separation. After the process, the separated components remain in the upper buffer, making them easy to collect for further analysis, such as mass spectrometry or LC/MS.
Main Function of the Agilent 3100 OFFGEL
In proteomics experiments, the Agilent 3100 OFFGEL is primarily used for the pre-fractionation of protein samples based on their isoelectric points. This process greatly simplifies the sample complexity, leading to improved detection sensitivity and analytical accuracy. It works well with three core technologies in proteomics: two-dimensional electrophoresis, mass spectrometry, and computer-assisted image analysis. Depending on the kit used, the system can achieve a pH resolution of 0.1–0.6, enabling precise separation and recovery of protein components for subsequent LC/MS studies.
We offer a wide range of ELISA kits, including imported ELISA kits, serum reagents, gelatin zymography test kits, rat ELISA test kits, and human ELISA test kits. Our products are sourced from trusted suppliers and are ideal for research in biochemistry, immunology, and molecular biology. For high-quality ELISA solutions, choose Nanjing Xinfan Biotechnology Co., Ltd.
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